Scientific Engineer
Scientific Engineer
PhD student
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Evaluation of hemostatic dressing in a groin puncture model in swine |
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The flexiVent is widely regarded as the gold standard for in vivo lung function measurements |
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Animal models of athero- and arteriosclerosis, arterial hypertension, myocardial infarction, hypetrophy of heart, diabetes mellitus, acid-base imbalance, denervation, acute hemorrhage, etc; fresh tumor cells inoculation (transplantaition) to rat, mice) |
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Restriction fragment length polymorphism analysis |
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Culture of neonatal cardiomyocytes |
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Western-blotting |
Real-time PCR |
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Electron and Fluorescence microscopic techniques (determination of apoptosis, necrosis, autophagy etc) |
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Single-cell reverse transcription real-time PCR |
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Enzymes’ activity determination using specific chromogenic and fluorogenic substrates (activities of proteasome, caspases, cathepsins, elastase, thrombin, plasmin, MMP, NOS, etc.) |
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RNA-interference |
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Immunohistochemical using specific monoclonal antibodies (immuno-fluorescent method) and specific fluorescent dyes. |
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Determination of histone acetylation status by chromatin immunoprecipitation |
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Fractionation of cells and subcellular particles using Percoll |
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Statistic analysis |
Gurianova V, Stroy D, Kruzliak P, Kyrichenko V, Moibenko A, Dosenko V. Does proteasome regulate the level of microRNA-1 in cardiomyocytes? Application to anoxia-reoxygenation // Mol Cell Biochem. 2015 Jun;404(1-2):45-51.
Orlovsky MA, Dosenko VE, Spiga F, Skibo GG, Lightman SL.Hippocampus remodeling by chronic stress accompanied by GR, proteasome and caspase-3 overexpression. Brain Res. 2014 Dec 17;1593:83-94.
Drevytska TI, Nagibin VS, Gurianova VL, Kedlyan VR, Moibenko AA, Dosenko VE.Silencing of TERT decreases levels of miR-1, miR-21, miR-29a and miR-208a in cardiomyocytes. Cell Biochem Funct. 2014 Oct;32(7):565-70.
Drozdovska SB, Dosenko VE, Ahmetov II, Ilyin VN.The association of gene polymorphisms with athlete status in ukrainians. Biol Sport. 2013 Sep;30(3):163-7.
Klymenko K, Novokhatska T, Kizub I, Parshikov A, Dosenko V, Soloviev A.PKC-δ isozyme gene silencing restores vascular function in diabetic rat. J Basic Clin Physiol Pharmacol. 2014 Jan 27:
Novokhatska T, Tishkin S, Dosenko V, Boldyriev A, Ivanova I, Strielkov I, Soloviev A.Correction of vascular hypercontractility in spontaneously hypertensive rats using shRNAs-induced delta protein kinase C gene silencing. Eur J Pharmacol. 2013 Oct 15;718(1-3):401-7. doi: 10.1016/j.ejphar.2013.08.003. Epub 2013 Aug 22.
Kyrychenko VO, Nagibin VS, Tumanovska LV, Pashevin DO, Gurianova VL, Moibenko AA, Dosenko VE, Klionsky DJ. Knockdown of PSMB7 induces autophagy in cardiomyocyte cultures: possible role in endoplasmic reticulum stress.Pathobiology. 2014;81(1):8-14.
Bačová B, Radošinská J, Viczenczová C, Knezl V, Dosenko V, Beňova T, Navarová J, Gonçalvesová E, van Rooyen J, Weismann P, Slezák J, Tribulová N. Up-regulation of myocardial connexin-43 in spontaneously hypertensive rats fed red palm oil is most likely implicated in its anti-arrhythmic effects. Can J Physiol Pharmacol. 2012 Sep;90(9):1235-45. (0,857)
Benova T, Viczenczova C, Radosinska J, Bacova B, Knezl V, Dosenko V, Weismann P, Zeman M, Navarova J, Tribulova N. Melatonin attenuates hypertension-related proarrhythmic myocardial maladaptation of connexin-43 and propensity of the heart to lethal arrhythmias // Can J Physiol Pharmacol. 2013 Aug;91(8):633-9.
Dosenko VE, Gurianova VL, Surova OV, Stroy DA, Moibenko AA. Mature and immature microRNA ratios in cultured rat cardiomyocytes during anoxia-reoxygenation. Exp Clin Cardiol. 2012 Summer;17(2):84-7. (0,576)
Drevytska T, Gavenauskas B, Drozdovska S, Nosar V, Dosenko V, Mankovska I. HIF-3α mRNA expression changes in different tissues and their role in adaptation to intermittent hypoxia and physical exercise. Pathophysiology. 2012 Jun;19(3):205-14.
Klionsky Daniel J. and 1269 others Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy. 2012 Vol. 8, Issue 4 (7,453)
Klionsky, Daniel J.; Abdelmohsen, Kotb; Abe, Akihisa; et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) // AUTOPHAGY (2016) V. 12, # 1, P. 1-222.
Kyrychenko S, Tishkin S, Dosenko V, Ivanova I, Novokhatska T, Soloviev A. The BK(Ca) channels deficiency as a possible reason for radiation-induced vascular hypercontractility. Vascul Pharmacol. 2012 Mar;56(3-4):142-9. (1,986)
Lisovyy O.O., Dosenko V.E., Nagibin V.S., Tumanovska L.V., Korol M.O., Surova O.V., Moibenko O.O. Cardioprotective effect of 5-lipoxygenase gene (ALOX5) silencing in ischemia-reperfusion // Acta Biochim Pol. – 2009. – V. 56. – №4. – P.687-94.
Lushnikova I. Orlovsky M. Dosenko V. Maistrenko A. Skibo D. Brief anoxia preconditioning and HIF prolyl-hydroxylase inhibition enhances neuronal resistance in organotypic hippocampal slices on model of ischemic damage// Brain Res. 2011. V. 1386. P.175-183
Panasiuk O., A. Shysh, A. Bondarenko, A. Moibenko. Omega-3 PUFA-enriched diet differentially protects two subpopulations of myocardial mitochondria against Ca2+-induced injury // Experimental and Clinical Cardiology – 2013. – Vol.18, № 1. – P. e60-e64.
Pashevin DA, Tumanovska LV, Dosenko VE, Nagibin VS, Gurianova VL, Moibenko AA. Antiatherogenic effect of quercetin is mediated by proteasome inhibition in the aorta and circulating leukocytes. – Pharmacol Rep. 2011;63(4):1009-18
Pashevin, Denis O.; Nagibin, Vasyl S.; Tumanovska, Lesya V.; et al. Proteasome Inhibition Diminishes the Formation of Neutrophil Extracellular Traps and Prevents the Death of Cardiomyocytes in Coculture with Activated Neutrophils during Anoxia-Reoxygenation // PATHOBIOLOGY (2015) V. 82, # 6, P. 290-298
Radosinska J, Bacova B, Bernatova I, Navarova J, Zhukovska A, Shysh A, Okruhlicova L, Tribulova N. Myocardial NOS activity and connexin-43 expression in untreated and omega-3 fatty acids-treated spontaneously hypertensive and hereditary hypertriglyceridemic rats // Mol Cell Biochem. – 2011. – N.347(1-2). – P.163-73.
Voitychuk O.I., Strutynskyi R.B., Moibenko O.O., Shuba Y.M. Effects of fluorine-containing opener of ATP-sensitive potassium channels, pinacidil-derivative flocalin, on cardiac voltage-gated sodium and calcium channels // NSAP. – 2012.-V.385. -№11. – Р. 1095-1102. (2,7)
Voitychuk O.I., Strutynskyi R.B., Yagupolskii L.М., Tinker A., Moibenko O.O., Shuba Y.M. Sarcolemmal cardiac KATP channels as a target for the cardioprotective effects of the fluorine-containing pinacidil analogue flocalin // Brit J Pharmacol. – 2011.-V.162. -№3. –Р. 701-711.
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